Production of inulinase from Kluyveromyces marxianus using Dahlia tuber extract
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چکیده
منابع مشابه
Production of inulinase from Kluyveromyces marxianus using dahlia tuber extract
Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL(-1)) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL(-1)). The yeast showed good growth on a simple medium containing dahlia ext...
متن کاملOPTIMIZATION OF INULINASE PRODUCTION USING COPRA WASTE BY Kluyveromyces marxianus var. marxianus
Kluyveromyces marxianus var. marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase production using copra waste as a carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nutrients for their influence on inulinase production was achieved using a Plackett– –Burman design...
متن کاملEffects of Culture Conditions on the Production of Inulinase by Kluyveromyces marxianus
The present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL) was obtained at a sucrose concentration of 10 g L, pH 5.0 and aeration rate of 1 vvm. The 20 g L sucrose concentration was suitable for...
متن کاملEffects of carbon sources, oxygenation and ethanol on the production of inulinase by Kluyveromyces marxianus YX01
Article info: Received: 16 July 2012 In revised form: 25 September 2012 Accepted: 29 October 2012 ABSTRACT Inulinase is one of the most important factors in consolidated bioprocessing, which combines enzyme production, inulin saccharification, and ethanol fermentation into a single process. In our study, inulinase production and cell growth of Kluyveromyces marxianus YX01 under different condit...
متن کاملIntrinsic Activity of Inulinase from Kluyveromyces marxianus ATCC 16045 and Carbon and Nitrogen Balances
Kluyveromyces marxianus ATCC 16045 was cultivated in a batch on minimal medium to overproduce inulinase. The fermented broth was purified by fast protein liquid chromatography and ultrafiltration, and a pure enzyme fraction was obtained. SDS-PAGE electrophoresis allowed calculating molecular mass of 59 kDa, while nitrogen determination by the micro-Kjeldahl method allowed evaluating intrinsic i...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2012
ISSN: 1517-8382
DOI: 10.1590/s1517-83822012000100007